Database Research Summaries2018 Improved Resistance to Soybean Cyst Nematode via Rhg1 and other Soybean loci
Research Focus
The focus of this project is to increase the understanding of Rhg1, the most important SCN resistance gene, to improve its effectiveness in commercially grown varieties.
Objectives
Identify and utilize two additional genes from wild soybean that confer improved SCN resistance.
Results
- Our yeast two-hybrid experiments have revealed two syntaxin homeologs that interact with the Rhg1 alpha-SNAP protein. Significantly, both of the genes encoding these proteins correlate strongly with known nematode resistance QTL. We have also detected interaction partners with the wound-inducible protein homolog at Rhg1, and have identified interaction partners of this protein with proteins from Hg type 0 SCN by using yeast two hybrid screens.
- We continued to work with field grow-outs of the 18 selections from Fayette that have putative changes in Rhg1 copy number. The seed of these lines will be grown in yield tests next summer to determine whether copy number changes are associated with yield.
- We started to prepare a manuscript regarding our putative identification of the relevant gene at cqSCN006 that causes elevated SCN resistance. We are waiting for transgenic soybean plants with disrupted cqSCN006 and cqSCN007 genes to come back from the UW-Madison WCIC plant transformation facility.
- We also conducted experiments that demonstrate functional traits of the cqSCN006 gamma-SNAP protein and observed weaker versions of the resistance-associated traits observed for the Rhg1 alpha-SNAP protein.
Importance
This project has a number of advanced discoveries, that if verified could have significant impact in improving SCN resistance, particularly in building on sources currently available.
For more information about this research project, please visit the National Soybean Checkoff Research Database.
Funded in part by the soybean checkoff.
